AustralieFrV1, Main, Exploration, bibRecord, 004653

Hypomethylation of the IL17RC Promoter in Peripheral Blood Leukocytes is Not A Hallmark of Age-Related Macular Degeneration

Identifieur interne : 004653 ( Main/Exploration ); précédent : 004652; suivant : 004654

Hypomethylation of the IL17RC Promoter in Peripheral Blood Leukocytes is Not A Hallmark of Age-Related Macular Degeneration

Auteurs : Verity F. Oliver [États-Unis] ; Maria Franchina [Australie] ; Andrew E. Jaffe [États-Unis] ; Kari E. Branham [États-Unis] ; Mohammad Othman [États-Unis] ; John R. Heckenlively [États-Unis] ; Anand Swaroop [États-Unis] ; Betsy Campochiaro [États-Unis] ; Brendan J. Vote [Australie] ; Jamie E. Craig [Australie] ; Richard Saffery [Australie] ; David A. Mackey [Australie] ; Jiang Qian [États-Unis] ; Donald J. Zack [États-Unis, France] ; Alex W. Hewitt [Australie] ; Shannath L. Merbs [États-Unis]

Source :

Cell reports [ 2211-1247 ] ; 2013.

RBID : PMC:3926096

Descripteurs français

KwdFr :
- Adulte d'âge moyen, Dégénérescence maculaire (génétique), Dégénérescence maculaire (métabolisme), Dégénérescence maculaire (sang), Femelle, Humains, Leucocytes (métabolisme), Marqueurs biologiques (sang), Mâle, Méthylation de l'ADN, Récepteurs aux interleukines (génétique), Récepteurs aux interleukines (sang), Régions promotrices (génétique), Sujet âgé, Sujet âgé de 80 ans ou plus, Études cas-témoins.
MESH :
- génétique : Dégénérescence maculaire, Récepteurs aux interleukines.
- métabolisme : Dégénérescence maculaire, Leucocytes.
- sang : Dégénérescence maculaire, Marqueurs biologiques, Récepteurs aux interleukines.
- Adulte d'âge moyen, Femelle, Humains, Mâle, Méthylation de l'ADN, Régions promotrices (génétique), Sujet âgé, Sujet âgé de 80 ans ou plus, Études cas-témoins.

English descriptors

KwdEn :
- Aged, Aged, 80 and over, Biomarkers (blood), Case-Control Studies, DNA Methylation, Female, Humans, Leukocytes (metabolism), Macular Degeneration (blood), Macular Degeneration (genetics), Macular Degeneration (metabolism), Male, Middle Aged, Promoter Regions, Genetic, Receptors, Interleukin (blood), Receptors, Interleukin (genetics).
MESH :
- chemical , blood : Biomarkers, Receptors, Interleukin.
- blood : Macular Degeneration.
- genetics : Macular Degeneration, Receptors, Interleukin.
- metabolism : Leukocytes, Macular Degeneration.
- Aged, Aged, 80 and over, Case-Control Studies, DNA Methylation, Female, Humans, Male, Middle Aged, Promoter Regions, Genetic.

Abstract

SUMMARY

Age-related macular degeneration (AMD) is a leading cause of visual impairment worldwide. Aberrant DNA methylation within the promoter of IL17RC in peripheral blood mononuclear cells has recently been reported in AMD. To validate this association, we examined DNA methylation of the IL17RC promoter in peripheral blood. First, we used Illumina Human Methylation450 Bead Arrays, a widely-accepted platform for measuring global DNA methylation. Second, methylation status at multiple sites within the IL17RC promoter was determined by bisulfite pyrosequencing in two cohorts. Third, a methylation-sensitive QPCR-based assay was performed on a subset of samples. In contrast to previous findings, we did not find evidence of differential methylation between AMD cases and age-matched controls. We conclude that hypomethylation within the IL17RC gene promoter in peripheral blood is not suitable for use as a clinical biomarker of AMD. This study highlights the need for considerable replication of epigenetic association studies prior to clinical application.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3926096

DOI: 10.1016/j.celrep.2013.11.042
PubMed: 24373284
PubMed Central: 3926096

Affiliations:

Le document en format XML

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 Promoter in Peripheral Blood Leukocytes is Not A Hallmark of Age-Related Macular Degeneration</title>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Hypomethylation of the <italic>IL17RC</italic>
 Promoter in Peripheral Blood Leukocytes is Not A Hallmark of Age-Related Macular Degeneration</title>
<author><name sortKey="Oliver, Verity F" sort="Oliver, Verity F" uniqKey="Oliver V" first="Verity F" last="Oliver">Verity F. Oliver</name>
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<country xml:lang="fr" wicri:curation="lc">Australie</country>
<wicri:regionArea>Centre for Ophthalmology and Visual Science, University of Western Australia, Lions Eye Institute, Perth 6009</wicri:regionArea>
<wicri:noRegion>Perth 6009</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Qian, Jiang" sort="Qian, Jiang" uniqKey="Qian J" first="Jiang" last="Qian">Jiang Qian</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287</wicri:regionArea>
<wicri:noRegion>Maryland 21287</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zack, Donald J" sort="Zack, Donald J" uniqKey="Zack D" first="Donald J" last="Zack">Donald J. Zack</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287</wicri:regionArea>
<wicri:noRegion>Maryland 21287</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="A9">Department of Molecular Biology and Genetics, Department of Neuroscience, and Institute of Genetic Medicine, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Molecular Biology and Genetics, Department of Neuroscience, and Institute of Genetic Medicine, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287</wicri:regionArea>
<wicri:noRegion>Maryland 21287</wicri:noRegion>
</affiliation>
<affiliation wicri:level="4"><nlm:aff id="A10">Institut de la Vision, Université Pierre et Marie Curie, Paris 75012, FRANCE</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">France</country>
<wicri:regionArea>Institut de la Vision, Université Pierre et Marie Curie, Paris 75012</wicri:regionArea>
<wicri:noRegion>75012</wicri:noRegion>
<placeName><settlement type="city">Paris</settlement>
<region type="région" nuts="2">Île-de-France</region>
<settlement type="city">Paris</settlement>
</placeName>
<orgName type="university">Université Pierre-et-Marie-Curie</orgName>
</affiliation>
</author>
<author><name sortKey="Hewitt, Alex W" sort="Hewitt, Alex W" uniqKey="Hewitt A" first="Alex W" last="Hewitt">Alex W. Hewitt</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Centre for Ophthalmology and Visual Science, University of Western Australia, Lions Eye Institute, Perth 6009, AUSTRALIA</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Australie</country>
<wicri:regionArea>Centre for Ophthalmology and Visual Science, University of Western Australia, Lions Eye Institute, Perth 6009</wicri:regionArea>
<wicri:noRegion>Perth 6009</wicri:noRegion>
</affiliation>
<affiliation wicri:level="4"><nlm:aff id="A11">Centre for Eye Research Australia, University of Melbourne, Royal Victorian Eye & Ear Hospital, East Melbourne 3002, AUSTRALIA</nlm:aff>
<country xml:lang="fr" wicri:curation="lc">Australie</country>
<wicri:regionArea>Centre for Eye Research Australia, University of Melbourne, Royal Victorian Eye & Ear Hospital, East Melbourne 3002</wicri:regionArea>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Merbs, Shannath L" sort="Merbs, Shannath L" uniqKey="Merbs S" first="Shannath L" last="Merbs">Shannath L. Merbs</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Department of Ophthalmology, Wilmer Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, Maryland 21287</wicri:regionArea>
<wicri:noRegion>Maryland 21287</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">Cell reports</title>
<idno type="eISSN">2211-1247</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aged</term>
<term>Aged, 80 and over</term>
<term>Biomarkers (blood)</term>
<term>Case-Control Studies</term>
<term>DNA Methylation</term>
<term>Female</term>
<term>Humans</term>
<term>Leukocytes (metabolism)</term>
<term>Macular Degeneration (blood)</term>
<term>Macular Degeneration (genetics)</term>
<term>Macular Degeneration (metabolism)</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Promoter Regions, Genetic</term>
<term>Receptors, Interleukin (blood)</term>
<term>Receptors, Interleukin (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adulte d'âge moyen</term>
<term>Dégénérescence maculaire (génétique)</term>
<term>Dégénérescence maculaire (métabolisme)</term>
<term>Dégénérescence maculaire (sang)</term>
<term>Femelle</term>
<term>Humains</term>
<term>Leucocytes (métabolisme)</term>
<term>Marqueurs biologiques (sang)</term>
<term>Mâle</term>
<term>Méthylation de l'ADN</term>
<term>Récepteurs aux interleukines (génétique)</term>
<term>Récepteurs aux interleukines (sang)</term>
<term>Régions promotrices (génétique)</term>
<term>Sujet âgé</term>
<term>Sujet âgé de 80 ans ou plus</term>
<term>Études cas-témoins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Biomarkers</term>
<term>Receptors, Interleukin</term>
</keywords>
<keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Macular Degeneration</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Macular Degeneration</term>
<term>Receptors, Interleukin</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Dégénérescence maculaire</term>
<term>Récepteurs aux interleukines</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Leukocytes</term>
<term>Macular Degeneration</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dégénérescence maculaire</term>
<term>Leucocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Dégénérescence maculaire</term>
<term>Marqueurs biologiques</term>
<term>Récepteurs aux interleukines</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Aged</term>
<term>Aged, 80 and over</term>
<term>Case-Control Studies</term>
<term>DNA Methylation</term>
<term>Female</term>
<term>Humans</term>
<term>Male</term>
<term>Middle Aged</term>
<term>Promoter Regions, Genetic</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Adulte d'âge moyen</term>
<term>Femelle</term>
<term>Humains</term>
<term>Mâle</term>
<term>Méthylation de l'ADN</term>
<term>Régions promotrices (génétique)</term>
<term>Sujet âgé</term>
<term>Sujet âgé de 80 ans ou plus</term>
<term>Études cas-témoins</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>SUMMARY</title>
<p id="P2">Age-related macular degeneration (AMD) is a leading cause of visual impairment worldwide. Aberrant DNA methylation within the promoter of <italic>IL17RC</italic>
 in peripheral blood mononuclear cells has recently been reported in AMD. To validate this association, we examined DNA methylation of the <italic>IL17RC</italic>
 promoter in peripheral blood. First, we used Illumina Human Methylation450 Bead Arrays, a widely-accepted platform for measuring global DNA methylation. Second, methylation status at multiple sites within the <italic>IL17RC</italic>
 promoter was determined by bisulfite pyrosequencing in two cohorts. Third, a methylation-sensitive QPCR-based assay was performed on a subset of samples. In contrast to previous findings, we did not find evidence of differential methylation between AMD cases and age-matched controls. We conclude that hypomethylation within the <italic>IL17RC</italic>
 gene promoter in peripheral blood is not suitable for use as a clinical biomarker of AMD. This study highlights the need for considerable replication of epigenetic association studies prior to clinical application.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
<li>États-Unis</li>
</country>
<region><li>Victoria (État)</li>
<li>Île-de-France</li>
</region>
<settlement><li>Melbourne</li>
<li>Paris</li>
</settlement>
<orgName><li>Université Pierre-et-Marie-Curie</li>
<li>Université de Melbourne</li>
</orgName>
</list>
<tree><country name="États-Unis"><noRegion><name sortKey="Oliver, Verity F" sort="Oliver, Verity F" uniqKey="Oliver V" first="Verity F" last="Oliver">Verity F. Oliver</name>
</noRegion>
<name sortKey="Branham, Kari E" sort="Branham, Kari E" uniqKey="Branham K" first="Kari E" last="Branham">Kari E. Branham</name>
<name sortKey="Campochiaro, Betsy" sort="Campochiaro, Betsy" uniqKey="Campochiaro B" first="Betsy" last="Campochiaro">Betsy Campochiaro</name>
<name sortKey="Heckenlively, John R" sort="Heckenlively, John R" uniqKey="Heckenlively J" first="John R" last="Heckenlively">John R. Heckenlively</name>
<name sortKey="Jaffe, Andrew E" sort="Jaffe, Andrew E" uniqKey="Jaffe A" first="Andrew E" last="Jaffe">Andrew E. Jaffe</name>
<name sortKey="Merbs, Shannath L" sort="Merbs, Shannath L" uniqKey="Merbs S" first="Shannath L" last="Merbs">Shannath L. Merbs</name>
<name sortKey="Othman, Mohammad" sort="Othman, Mohammad" uniqKey="Othman M" first="Mohammad" last="Othman">Mohammad Othman</name>
<name sortKey="Qian, Jiang" sort="Qian, Jiang" uniqKey="Qian J" first="Jiang" last="Qian">Jiang Qian</name>
<name sortKey="Swaroop, Anand" sort="Swaroop, Anand" uniqKey="Swaroop A" first="Anand" last="Swaroop">Anand Swaroop</name>
<name sortKey="Zack, Donald J" sort="Zack, Donald J" uniqKey="Zack D" first="Donald J" last="Zack">Donald J. Zack</name>
<name sortKey="Zack, Donald J" sort="Zack, Donald J" uniqKey="Zack D" first="Donald J" last="Zack">Donald J. Zack</name>
</country>
<country name="Australie"><noRegion><name sortKey="Franchina, Maria" sort="Franchina, Maria" uniqKey="Franchina M" first="Maria" last="Franchina">Maria Franchina</name>
</noRegion>
<name sortKey="Craig, Jamie E" sort="Craig, Jamie E" uniqKey="Craig J" first="Jamie E" last="Craig">Jamie E. Craig</name>
<name sortKey="Hewitt, Alex W" sort="Hewitt, Alex W" uniqKey="Hewitt A" first="Alex W" last="Hewitt">Alex W. Hewitt</name>
<name sortKey="Hewitt, Alex W" sort="Hewitt, Alex W" uniqKey="Hewitt A" first="Alex W" last="Hewitt">Alex W. Hewitt</name>
<name sortKey="Mackey, David A" sort="Mackey, David A" uniqKey="Mackey D" first="David A" last="Mackey">David A. Mackey</name>
<name sortKey="Saffery, Richard" sort="Saffery, Richard" uniqKey="Saffery R" first="Richard" last="Saffery">Richard Saffery</name>
<name sortKey="Vote, Brendan J" sort="Vote, Brendan J" uniqKey="Vote B" first="Brendan J" last="Vote">Brendan J. Vote</name>
</country>
<country name="France"><region name="Île-de-France"><name sortKey="Zack, Donald J" sort="Zack, Donald J" uniqKey="Zack D" first="Donald J" last="Zack">Donald J. Zack</name>
</region>
</country>
</tree>
</affiliations>
</record>

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Hypomethylation of the IL17RC Promoter in Peripheral Blood Leukocytes is Not A Hallmark of Age-Related Macular Degeneration

Hypomethylation of the IL17RC Promoter in Peripheral Blood Leukocytes is Not A Hallmark of Age-Related Macular Degeneration

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